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l 190.2 205.2 l 430.32 436.32 l 284.64 614.52 l 'S 359.52 229.8 l 240.6 245.52 m f* 441 243 l 326.04 423.12 l 0.059 0.212 0.631 rg 387.36 297.24 l 0 0 0.498 rg 509.64 211.68 m 419.64 596.76 l -0.007 Tw 14.4 273.48 582.48 -5.4 re S 223.68 440.52 1.56 -0.06 re 1 g 192.36 408 l 356.76 229.32 1.56 -0.06 re 152.16 81.84 l f* S 157.32 553.56 l f* 455.04 228.12 l 194.4 232.08 1.68 -0.06 re 185.04 428.88 l 398.16 418.08 l 149.16 301.8 0.48 -0.06 re 308.097 582.48 306 584.577 306 587.16 c 437.16 535.08 l 386.4 251.88 l f* 470.04 414.72 0.36 -0.06 re 0 g f* 0 g 108.96 526.92 l 417.6 441.24 0.6 -0.06 re 266.16 526.08 l 191.04 554.4 l 183.84 598.721 176.905 591.84 168.36 591.84 c 196.56 511.2 l 178.08 580.8 l S 411.6 581.16 l 261.24 484.44 l f* 185.76 361.44 l 202.8 655.92 l 420.36 573.24 0.36 -0.06 re 0 g 381 172.08 l 405.48 173.28 l 462.48 318.96 l 356.52 542.88 m 304.56 246.84 l 131.64 545.28 l f* 242.88 251.16 l f* 299.64 545.76 l 317.88 246.72 l 295.92 566.16 l S 317.16 613.2 l S 443.4 467.88 m 191.52 530.88 l 291.48 229.92 1.68 -0.06 re f* 212.4 414.48 0.48 -0.06 re 257.04 611.16 l S S 14.4 513.36 582.48 -5.4 re 317.16 163.32 m S 195.6 508.44 l 1 g 204.24 580.68 m 328.56 435.12 l 219.96 339.24 l 218.16 316.68 l (\251 1994, 1995 Robert Robbins)Tj -0.001 Tc f* 330.72 524.64 l f* 404.337 267.84 408.96 263.217 408.96 257.52 c 447.72 562.44 l 152.16 626.88 l 277.44 302.16 0.6 -0.06 re 317.64 242.4 l 268.56 407.88 l 333.24 435.12 m 495 563.4 l 137.61 325.2 143.04 319.77 143.04 313.08 c 134.76 158.52 342.6 -4.8 re f* 121.56 303.72 l 348.12 204.48 l S f* 204.24 189.96 m ET 14.4 273.48 582.48 -5.4 re 377.04 272.64 l f* 247.92 544.08 l 354.72 312.96 l 0 g 287.16 297.24 l 218.4 233.52 l 291.12 145.56 6 -4.68 re S 0000316757 00000 n (analytical methods for assembling sequence fragments. @gf#lqe\Uaj;KmOTQZZgZB-&[uSp9bX;j"A%stm[RDW`R/0#%l/-P>8Tg!RqEYZ f* 240.6 149.52 l 406.68 228.72 l 515.52 341.28 l S S f* 451.56 426.12 l 383.28 267 l 189.36 226.2 1.56 -0.06 re 148.56 303.24 0.6 -0.06 re 164.16 403.32 m 9.96 0 0 9.96 107.52 625.32 Tm 275.4 303.72 0.48 -0.06 re 1 g 177.48 166.8 m 224.76 299.64 l 484.68 299.64 l 120.96 256.92 l 1 i 1 J 1 j 0.96 w 10 M []0 d 465.819 586.08 458.4 593.445 458.4 602.52 c f* 315.36 357.96 l 426.24 444.6 l 318.72 519.96 l 259.8 234.84 l 317.28 521.88 l 0.002 Tc 39.12 742.2 l 275.64 252.6 l 0 g f* 163.92 265.92 l 469.44 231.96 l 266.28 565.68 l 391.44 232.92 l 256.56 228.48 l 0 G 439.68 239.4 l 0 g 230.64 437.64 l 300.36 631.92 l 1 g 234 581.4 l 186.24 355.44 l 170.88 264.48 l 335.76 588.36 l 317.64 566.16 l 232.44 228.96 l 368.76 398.28 l 369.6 574.8 0.36 -0.06 re 166.8 572.16 l f* 192.24 226.32 1.44 -0.06 re 344.28 574.08 0.36 -0.06 re 329.76 228.84 m 304.8 144.48 l f* 202.8 267.48 l 289.92 441.12 0.48 -0.06 re (complementarity rules: A always pairs with T and C always pairs with G.)Tj 444.12 153.12 m 330.72 524.64 l 190.44 189.72 l 237.12 230.16 l 142.2 293.4 l 234.12 230.4 l 480.72 551.64 0.48 -0.06 re 418.32 182.28 l 371.76 300.36 2.52 -0.06 re 0 g 181.92 204.48 m 0.678 0.412 0 rg 317.52 245.64 l 1 g W n S 12.96 0 0 12.96 369.24 580.08 Tm 376.08 284.52 l 490.08 545.76 l 183.24 249.12 l 138.24 441.72 l 380.88 299.28 l 320.4 651.12 l (C)Tj 374.88 413.64 0.48 -0.06 re 439.68 238.32 l 505.2 526.92 l 357.12 293.88 l 324.6 562.56 l 310.56 241.56 l 99.72 216.6 l 470.52 560.52 l 261.24 566.16 m 387.36 407.76 m 87.129 84.72 84.12 87.729 84.12 91.44 c f* Q f* f* S 445.08 261.24 m f* 220.92 625.68 l S 268.56 480.84 l 14.4 513.36 582.48 -5.4 re 1 g Q 278.52 524.28 l f* f* 1 i 1 J 1 j 0.96 w 10 M []0 d 208.8 231.84 l 293.64 553.8 7.32 -5.04 re 478.08 271.8 l 310.56 565.56 l f* 131.64 394.92 l q 507.96 448.32 0.84 -0.06 re 157.08 265.92 1.56 -0.06 re 153.36 397.92 7.2 -2.52 re 126.12 441.6 0.48 -0.06 re 251.28 421.08 l 263.76 435.24 l 506.16 252.12 l 509.28 452.04 1.08 -0.06 re 439.32 236.88 l 155.16 261.12 1.44 -0.06 re 126.24 299.52 l 0 g f* 196.56 404.4 m 221.4 505.56 l 433.68 421.92 l W n 327.96 495.24 l 304.56 186.48 l 220.2 234.12 l 234 226.56 l W n 0000397163 00000 n 242.16 580.68 m f 404.4 301.32 0.72 -0.06 re S 305.88 189.96 l f* 400.32 425.16 l 419.04 546 l 0.031 0.114 0.345 rg f* f* f* 228 615.12 l 332.76 435.36 l 181.92 566.16 m 364.56 625.92 l f* 0.031 0.114 0.345 rg 170.88 289.56 l 0 Tr f* 360.72 627.6 l S 462.6 442.68 l 291.24 581.28 l 326.64 415.56 m 398.88 606.96 l 0 g W n /F12 1 Tf 253.68 508.44 l 1 i 1 J 1 j 2.04 w 10 M []0 d 208.8 581.4 l 215.88 145.32 l S 14.4 525.6 582.48 -56.28 re f* 315.96 408.84 l 215.28 451.8 l 406.44 600.96 l f* 365.64 564.6 l f* 398.16 420.12 l /F6 1 Tf 228.24 647.64 l 177.48 651.48 l 339.24 300.96 0.48 -0.06 re 445.68 408.6 l f* 132.96 437.52 l 281.64 532.68 l 334.8 246.12 l 258.72 526.44 l 284.64 234.12 l 423.48 564.6 l f* f* 498.24 285.72 0.96 -0.06 re 158.16 309.96 l 0.001 Tc W n 448.08 186.72 l f* 439.92 534.72 l 131.64 594.48 m 442.2 601.8 l f* 168 574.44 l 192.96 308.16 l (Robert J. 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